The invention provides a method of labeling of cellular glycans bearing azide group via a fluorescent labeling technique. The method entails generating fluorescent probe form a nonfluorescent precursor, 4-ethynyl-N-ethyl-1,8-naphthalimide, by Cu(I)-catalyzed [3+2] cycloaddition (click activation) of alkyne group of the probe with an azido-containing fucose analog into glycoprotein via the fucose salvage pathway. Cells treated with 6-azidofucose are labeled with the click activated fluorogenic probe or biotinylated alkyne.
Powerful technique for visualizing the intracellular localization of fucosylated glycoconjuates by fluorescent microscopy
Glycoprotein imaging Glycoprotein purification Identification of key biomarkers Cancer Research Diagnosis of Disease
Chi-Huey Wong, Tsui-Ling Hsu, Masaaki Sawa, Sarah R Hanson
Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo